PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Palma J.M., Lisboa R.C., Rodrigues D.P., Santos A.F.M., Hofer E. & Santana A.P. 2016. [Molecular characterization of Listeria monocytogenes from beef samples and cattle slaughterhouses located in the Federal District, Brazil.] Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil. Pesquisa Veterinária Brasileira 36(10):957-964. Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF 70910-900, Brazil. E-mail: joanamarchesini@gmail.com The aim of the study was the analysis of Listeria monocytogenes strains in beef samples as well as slaughterhouse environment, located in the Federal District, promote serotyping by polymerase chain reaction (PCR), perform antibiotic susceptibility and submit the strains to Pulsed-field gel electrophoresis (PFGE). A total of 125 beef samples were analyzed, 45 samples of carcasses swabs and 43 swab samples. It detected 13 strains of Listeria monocytogenes, 11 in beef samples. and 2 in slaughterhouse environment. No carcass swabs strains were isolated. Among the 13 strains of L. monocytogenes six strains of serotype 4b were found, five serotype 1/2c and two strains of serotype 1/2a. Among the 11 strains of L. monocytogenes found in beef, one (9.1%) strain showed resistance to erythromycin, one (9.1%) strain to gentamicin, one to ciprofloxacin (9.1%) and all strains (100%) were resistant to nalidixic acid. The two strains coming from the slaughterhouse drains, all (100%) were resistant to nalidixic acid and Sulfonamides. The analysis by pulsed field gel electrophoresis (PFGE) showed 13 different pulsotypes; they were grouped into three different clonal groups, coincidentally correlated with the three different serotypes found, what suggests a widespread dissemination of these profiles in the Federal District, Brazil.

• Listeria monocytogenes listeriosis meat eletroforese listeriose

Abstract in Portuguese:

RESUMO.- Palma J.M., Lisboa R.C., Rodrigues D.P., Santos A.F.M., Hofer E. & Santana A.P. 2016. [Molecular characterization of Listeria monocytogenes from beef samples and cattle slaughterhouses located in the Federal District, Brazil.] Caracterização molecular de Listeria monocytogenes oriundas de cortes cárneos bovinos e de abatedouros frigoríficos de bovinos localizados no Distrito Federal, Brasil. Pesquisa Veterinária Brasileira 36(10):957-964. Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF 70910-900, Brazil. E-mail: joanamarchesini@gmail.com Este trabalho teve como objetivo realizar a detecção de cepas de Listeria monocytogenes de cortes cárneos bovinos bem como no ambiente de abatedouros frigoríficos localizados no Distrito Federal, promover a sorotipificação pela reação em cadeia da polimerase (PCR), realizar antibiograma e submeter às cepas à eletroforese de campo pulsado (Pulsed-field gel electrophoresis - PFGE). Foram analisados um total de 125 cortes cárneos bovinos, 45 amostras de swabs de carcaças e 43 amostras de swabs em que foram detectados 13 cepas de Listeria monocytogenes, sendo 11 em cortes cárneos bovinos e 2 swabs de ambiente em um abatedouro frigorifico. Não foram isoladas cepas de swabs de carcaça. Dentre as 13 cepas de Listeria monocytogenes foram encontradas seis cepas do sorotipo 4b, cinco do sorotipo 1/2c e duas cepas do sorotipo 1/2a. Dentre as 11 cepas de L. monocytogenes encontradas em cortes cárneos bovino, uma (9,1%) cepa apresentou resistência a eritromicina, outra (9,1%) cepa a gentamicina e outra a ciprofloxacina (9,1%) e todas as cepas (100%) apresentaram resistência ao Ác. Nalidíxico. Das duas (2) cepas oriundas de ralos de abatedouro frigorífico, todas (100%) apresentaram resistência ao Ác. Nalidíxico e a sulfonamidas. A análise por eletroforese de campo pulsante (PFGE) demonstrou 13 diferentes pulsotipos, em que foram agrupados em 3 diferentes grupos clonais, que coincidentemente se correlacionavam com os 3 diferentes sorotipos encontrados sugerindo uma ampla disseminação desses perfis no Distrito Federal.

Abstract in English:

Hofer E. & Reis C.M.F. 2005. [Species and serovars of Listeria isolated from sick and clinically healthy animals in Brazil.] Espécies e sorovares de Listeria isolados de animais doentes e portadores no Brasil. Pesquisa Veterinária Brasileira 25(2):79-83. Laboratório de Zoonoses Bacterianas, Depto Bacteriologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ 21045-900, Brazil. E-mail: ehofer@ioc.fiocruz.br Two hundred fourty-six strains of the genus Listeria were isolated from sick and clinically healthy animals, collected in three different regions of Brazil during 1971-2000. About 88.2% (217 cultures) yielded Listeria species from faecal specimens of healthy cattle and 29 strains (11.7%) were isolated from sick animals: 15 (6.0%) from central nervous system (CNS) and 14(5.6%) were from otherwise sterile sites. Phenotyping techniques were used to characterize the Listeria isolates. The commonest were L. innocua 6a and non-typable (140/56.9%), L. monocytogenes 4a (37/15.0%) and 4b (22/8.9%), originated mainly from stools of healthy cattle. From sick animals the predominant species and serovars were L. monocytogenes 4b (14/5.6%), and the higher incidence was observed in ruminants (12/4.8%) and 8/3.2% of the serovar 1a from other animal species (rodents and canines) mainly isolated from CNS samples.

• Listeria species serovars sick and healthy animals.

Abstract in Portuguese:

Hofer E. & Reis C.M.F. 2005. [Species and serovars of Listeria isolated from sick and clinically healthy animals in Brazil.] Espécies e sorovares de Listeria isolados de animais doentes e portadores no Brasil. Pesquisa Veterinária Brasileira 25(2):79-83. Laboratório de Zoonoses Bacterianas, Depto Bacteriologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ 21045-900, Brazil. E-mail: ehofer@ioc.fiocruz.br Two hundred fourty-six strains of the genus Listeria were isolated from sick and clinically healthy animals, collected in three different regions of Brazil during 1971-2000. About 88.2% (217 cultures) yielded Listeria species from faecal specimens of healthy cattle and 29 strains (11.7%) were isolated from sick animals: 15 (6.0%) from central nervous system (CNS) and 14(5.6%) were from otherwise sterile sites. Phenotyping techniques were used to characterize the Listeria isolates. The commonest were L. innocua 6a and non-typable (140/56.9%), L. monocytogenes 4a (37/15.0%) and 4b (22/8.9%), originated mainly from stools of healthy cattle. From sick animals the predominant species and serovars were L. monocytogenes 4b (14/5.6%), and the higher incidence was observed in ruminants (12/4.8%) and 8/3.2% of the serovar 1a from other animal species (rodents and canines) mainly isolated from CNS samples.

Abstract in English:

Lázaro N.S., Tibana A., Rodrigues D.P., Reis E.M.F., Quintaes B.R. & Hofer E. 2004. Antimicrobial resistance and R-plasmid in Salmonella spp from swine and abattoir environments. Pesquisa Veterinária Brasileira 24(2):57-60. Depto Epidemiologia e Saúde Pública, Instituto de Veterinária, UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: nslazaro@aol.com Salmonella serovars isolated from swine are of particular interest not only because of the pathogenic potential for this animal species, but also due to its relevance with regard to public health. On basis of the profile of resistance to antimicrobials, 13 Salmonella strains were selected which belonged to the serovars Muenster (7), Derby (4), Typhimurium (1), and Braenderup (1). They were isolated from healthy swine as well as from the abattoir environment in the state of Rio de Janeiro. All strains of Salmonella were subjected to bacterial conjugation, and the E. coli K12 Nalr Lac+ F standard strain was used as receptor, with the purpose to verify the ability to transfer the resistance marks. Gene transfer phenomenon was detected in seven strains, and except Salmonella Typhimurium which transconjugated to Sm, Tc and Su, the remaining ones were characterized by transferring mark Su only. By plasmidial analysis of strains used and their respective transconjugants, 63 Kb plasmid was found, which was probably related to S. Typhimurium resistance.

• Salmonella swine antimicrobial resistance R-plasmid.

Abstract in Portuguese:

Lázaro N.S., Tibana A., Rodrigues D.P., Reis E.M.F., Quintaes B.R. & Hofer E. 2004. Antimicrobial resistance and R-plasmid in Salmonella spp from swine and abattoir environments. Pesquisa Veterinária Brasileira 24(2):57-60. Depto Epidemiologia e Saúde Pública, Instituto de Veterinária, UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: nslazaro@aol.com Salmonella serovars isolated from swine are of particular interest not only because of the pathogenic potential for this animal species, but also due to its relevance with regard to public health. On basis of the profile of resistance to antimicrobials, 13 Salmonella strains were selected which belonged to the serovars Muenster (7), Derby (4), Typhimurium (1), and Braenderup (1). They were isolated from healthy swine as well as from the abattoir environment in the state of Rio de Janeiro. All strains of Salmonella were subjected to bacterial conjugation, and the E. coli K12 Nalr Lac+ F standard strain was used as receptor, with the purpose to verify the ability to transfer the resistance marks. Gene transfer phenomenon was detected in seven strains, and except Salmonella Typhimurium which transconjugated to Sm, Tc and Su, the remaining ones were characterized by transferring mark Su only. By plasmidial analysis of strains used and their respective transconjugants, 63 Kb plasmid was found, which was probably related to S. Typhimurium resistance.

Abstract in English:

Lázaro N.S., Tibana A., Reis E.M.F., Rodrigues D.P., Quintaes B.R. & Hofer E. 2004. [Antimicrobial susceptibility pattern and plasmid profile in Salmonella Muenster isolated from swine and abattoir environment, Brazil.] Padrão de susceptibilidade a antimicrobianos e perfil plasmidial em Salmonella Muenster isoladas de suínos e do ambiente de abatedouros. Pesquisa Veterinária Brasileira 24(2):65-70. Depto Epidemiologia e Saúde Pública, Inst.Veterinária, UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: nslazaro@aol.com Thirty-eight strains of Salmonella Muenster, isolated from swine and the abattoir environment, in the State of Rio de Janeiro, Brazil, from March 1991 to February 1992, were analyzed for the presence of plasmids. The strains were selected according to their profile regarding the antimicrobials: streptomycin, tetracycline, sulphonamide and sulfametoxazole-trimethoprim. Thirteen strains were resistant to one or several antimicrobials, 18 with intermediate degree and seven were sensitive. Plasmids varying in size from 1.2 Kb to 42 Kb were detected in 37 (97.36%) of the 38 samples, corresponding to 11 different profiles (P1- P11), varying from 1 to 6 plasmids per model. The number and plasmids diversity was greater than the resistance marks for strains. The plasmid of 2.85 Kb was the most frequent, being present in 83.78% of the 37 strains; only the one of 7.5 Kb was detected at the two slaughterhouses. There was no parallelism between resistance pattern and plasmidial profile, and a same antibiotype was found in several plasmidial profiles. The results of the present investigation, allowed us to conclude that the plasmid characterization is an useful and simple tool for the epidemiological typing of this sorovar.

• Salmonella Muenster swine antimicrobial resistance plasmids.

Abstract in Portuguese:

Lázaro N.S., Tibana A., Reis E.M.F., Rodrigues D.P., Quintaes B.R. & Hofer E. 2004. [Antimicrobial susceptibility pattern and plasmid profile in Salmonella Muenster isolated from swine and abattoir environment, Brazil.] Padrão de susceptibilidade a antimicrobianos e perfil plasmidial em Salmonella Muenster isoladas de suínos e do ambiente de abatedouros. Pesquisa Veterinária Brasileira 24(2):65-70. Depto Epidemiologia e Saúde Pública, Inst.Veterinária, UFRRJ, Seropédica, RJ 23890-000, Brazil. E-mail: nslazaro@aol.com Thirty-eight strains of Salmonella Muenster, isolated from swine and the abattoir environment, in the State of Rio de Janeiro, Brazil, from March 1991 to February 1992, were analyzed for the presence of plasmids. The strains were selected according to their profile regarding the antimicrobials: streptomycin, tetracycline, sulphonamide and sulfametoxazole-trimethoprim. Thirteen strains were resistant to one or several antimicrobials, 18 with intermediate degree and seven were sensitive. Plasmids varying in size from 1.2 Kb to 42 Kb were detected in 37 (97.36%) of the 38 samples, corresponding to 11 different profiles (P1- P11), varying from 1 to 6 plasmids per model. The number and plasmids diversity was greater than the resistance marks for strains. The plasmid of 2.85 Kb was the most frequent, being present in 83.78% of the 37 strains; only the one of 7.5 Kb was detected at the two slaughterhouses. There was no parallelism between resistance pattern and plasmidial profile, and a same antibiotype was found in several plasmidial profiles. The results of the present investigation, allowed us to conclude that the plasmid characterization is an useful and simple tool for the epidemiological typing of this sorovar.

Abstract in English:

ABSTRACT.- Hofer E., Zamora M.R.N., Lopes A.E., Moura A.M.C:, Araújo H.L., Leite M.D.D. & Silva Filho S.J. 2000. [Salmonella serovars in meat of horses slaughtered in northeastern Brazil.] Sorovares de Salmonella. em carne de eqüídeos abatidos no nordeste do Brasil. Pesquisa Veterinária Brasileira 20(2):80-84. Depto Bacteriologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de janeiro, RJ 21045-900, Brazil. In the sixties and seventies there was an extraordinary increase in export of horse meat products to Europe andjapah. This favored an increase in risk of Salmonella outspread through those products to human and animal consumer populations. Thus, from an exporting company dealing with horse meat located in northeastern Brazil (state of Pernambuco), 19,238 fragments of more external muscles, Salmonella was isolated from 666 samples colleted from 433 animals (horses and donkeys). The serotyping of 745 isolates showed 98 serovars pertaining to 14 serogroups, predominantly classified into subspecies 1 (98.9%). S. Anatum, S. Carrau, S. Saintpaul, S. Agona, and S. Typhimurium were the most frequent serovars isolated. Preliminary data indicate that the primary causes for the presence of Salmonella in the meats probably was contact with feces from slaughtered animals, as well a.s possible contamination of environments, in view of the absence of human carriers researched in part of the personnel.

• Horses horse meat Salmonella serogroups serovars Eqüídeos produtos cárneos sorogrupos sorovares.

Abstract in Portuguese:

SINOPSE.- Hofer E., Zamora M.R.N., Lopes A.E., Moura A.M.C:, Araújo H.L., Leite M.D.D. & Silva Filho S.J. 2000. [Salmonella serovars in meat of horses slaughtered in northeastern Brazil.] Sorovares de Salmonella. em carne de eqüídeos abatidos no nordeste do Brasil. Pesquisa Veterinária Brasileira 20(2):80-84. Depto Bacteriologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de janeiro, RJ 21045-900, Brazil. Nas décadas de 60 e 70, houve um extraordinário incremento da exportação de produtos cárneos de equídeos dos países da América do Sul para a Europa e Japão. Este acontecimento favoreceu o aumento de risco da veiculação de Salmonella através desses produtos, para as populações humana e animal, consumidoras. Assim, num estabelecimento industrial e exportador de carne de eqüídeos localizado no nordeste do Brasil (Pernambuco), foram analisados bacteriologicamente, 19.238 fragmentos de músculos mais externos, que revelaram 666 exames positivos referentes a 433 animais (eqüinos e asininos) e resultando no isolamento de 745 cepas de Salmonella. Na amostragem foram·caracterizados do ponto de vista antigénico 98 sorovares, predominantemente classificados na subespécie 1 (98,9%) e tendo como os mais freqüentes S. Anatum, S. Carrau, S. Saintpaul, S. Agona e S. Typhimurium. Pelas análises efetuadas admite-se que as causas primordiais da presença de Salmonella nas carnes, provavelmente decorreu do contato com os excretas dos animais abatidos, bem como pela possível contaminação ambiental resultante, tendo em vista a ausência de portadores humanos, pesquisados numa parcela do pessoal.

Abstract in English:

ABSTRACT.- Hofer E., Silva Filho S.J. & Reis E.M.F. 1998. [Salmonella serovars isolated from feedstuff and poultry feeds in Brazil.] Sorovares de Salmonella isolados de matérias-primas e de ração para aves no Brasil. Pesquisa Veterinária Brasileira 18(1):21-27. Depto Bacteriologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ 21045-900, Brazil. Salmonella strains were isolated from feedstuff and poultry feeds from several regions of Brazil in 1976 and from 1979 to 1991. Serotyping of 2293 isolates showed 151 serovars which pertained to 17 serogroups and were classified as subspecies I (99.6%), III (0.33%) and IV (0.04%). There was a predominance of groups 0:7 (30.4%), 0:4 (24.5%), 0:3, 10 (19.1%), 0:13 (7 .8%), 0:1, 3, 19 (4.9%) and 0:18 (3. 7%), representing 90% of the serogroups characterized that accounted for 103 different serotypes (68.2%). Predominant serovars isolated from all sources were S. Montevideo, S. Senftenberg, S. Havana, S. Mbandaka, S. Tennessee, S. Infantis, S. Agona, S. Anatum, S. Cerro and S. Bredeney. Bacteriological and epidemiological aspects and the relationship with serovars isolated from poultry are discussed.

• Poultry feeds Salmonella serogroup serovar prevalence Matéria-prima ração aves sorogrupos sorovares.

Abstract in Portuguese:

RESUMO.- Hofer E., Silva Filho S.J. & Reis E.M.F. 1998. [Salmonella serovars isolated from feedstuff and poultry feeds in Brazil.] Sorovares de Salmonella isolados de matérias-primas e de ração para aves no Brasil. Pesquisa Veterinária Brasileira 18(1):21-27. Depto Bacteriologia, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ 21045-900, Brazil. Foram caracterizadas antigenicamente amostras de Salmonella isoladas de matérias-primas e de ração para aves em 1976 e durante doze anos consecutivos (1979-1991). As 2293 culturas analisadas provieram de sete regiões distintas do país e possibilitaram o reconhecimento de 151 sorovares, classificados bioquimicamente nas subespécies I (99,6%) IIIa (0,33%) e IV (0,04%), respectivamente. Os sorovares identificados se distribuiram por 17 sorogrupos, com predominância de 0:7 (30,4%), 0:4 (24,5%), 0:3, 10 (19, 1%), 0:13 (7,8%), 0:1,3,19 (4,9%) e 0:18 (3,7), que representam 90% dos grupos sorológicos caracterizados e constituídos de 103 (68,2%) sorotipos. Dentre os dez sorovares mais frequentemente reconhecidos citam-se S. Montevideo, S. Senftenberg, S. Havana, S. Mbandaka, S. Tennessee, S. Infantis, S. Agona, S. Anatum, S. Cerro e S. Bredeney. Alguns aspectos de caráter epidemio-lógico foram discutidos, envolvendo particularmente, determinados sorotipos e·inclusive confrontando-se os resultados obtidos com aqueles oriundos de investigação conexa em aves.

Abstract in English:

Salmonella strains were isolated from ill and shedding birds in several regions of Brazil between 1962 and 1991. Serotyping of 2123 isolates showed 90 serovars pertaining to 14 serogroups. There was a predominance of groups 0:9 (40.0%), 0:4 (33.3%), 0:7 (10.6%) and 0:3, 10 (6.7%). Major serovar diversity was found to serogroup 0:7 that accounted for 22 different types, followed by serogroups 0:4, 0:3, 10 and 0:9 with 19, 15 and 10 serotypes respectively. An average of 10.8 serovars was isolated per year. S. Gallinarum, S. Pullorum, S. Typhimurium, S. Heidelberg, S. Enteritidis and S. Infantis were the most frequent serovars found over the 30 years, representing 65% to 67% of the total of isolates. Bacteriological and epidemiological aspects concerning a number of serotypes are discussed.

• Birds Salmonella prevalence serogroups serovars

Abstract in Portuguese:

Foram caracterizadas antigenicamente amostras de Salmonella isoladas de aves (portadoras e doentes) provenientes de diversas regiões do país durante o período de 1962 a 1991. Nas 2123 culturas analisadas foram reconhecidos 90 sorovares, distribuídos em 14 sorogrupos com predominância dos grupos 0:9 (40,0%), 0:4 (33,3%), 0:7 (10,6%) e O: 3, 10 (6,7%). A maior diversidade de sorovares foi reconhecida no sorogrupo 0:7 com 22 tipos distintos, secundado por 0:4, 0:3,10 e 0:9, constituídos de 19, 15 e 10 sorotipos, respectivamente. No computo geral, foi determinada a média de 10,8 sorovares isolados por ano. Os sorovares classificados como muito frequentes nos três decênios, representando 65 a 67%, dos isolamentos, foram S. Gallinarum, S. Pullorum, S. Typhimurium, S. Heidelberg, S. Enteritidis e S. Infantis. Considerações de natureza bacteriológica e epidemiológica foram discutidas em relação a alguns dos sorotipos prevalentes.

• Aves Salmone/la prevalência sorogrupos sorovares

Abstract in English:

ABSTRACT.- Lázaro N.S. & Hofer E. 1997.Anti-Yersinia enterocolitica serotype 3 agglutinins in swine sera from Rio de Janeiro.[Aglutininas anti-Yersinia enterocolitica 0:3 em soros de suínos do Rio de Janeiro.] Pesquisa Veterinária Brasileira 17(1):9-11. Depto Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23851-970, Brazil. The slow serum agglutination test was applied to 119 healthy pigs for the determination of the possible presence of anti-Yersinia enterocolitica 0:3 agglutinins. Of the 63.9% reactive animals (≥ 1 :20), 8.4% presented positive titers (≥1 :80), suggesting the presence of this pathogen among swine and consequently an additional public health problem.

• Yersinia enterocolitica agglutinins swine aglutininas suíno.

Abstract in Portuguese:

SINOPSE.- Lázaro N.S. & Hofer E. 1997.Anti-Yersinia enterocolitica serotype 3 agglutinins in swine sera from Rio de Janeiro.[ Aglutininas anti-Yersinia enterocolitica 0:3 em soros de suínos do Rio de Janeiro.] Pesquisa Veterinária Brasileira 17(1):9-11. Depto Epidemiologia e Saúde Pública, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23851-970, Brazil. Aglutininas anti-Yersinia enterocolitica 0:3 em soros de suínos do Rio de Janeiro. A pesquisa de aglutininas anti-Yersinia enterocolitica 0:3 foi realizada em 119 suínos sadios, através da prova de soro-aglutinação lenta. Dos 63,9% animais reagentes (≥1:20), 8,4% apresentaram títulos a nível de positivo (≥1:80) sugerindo a presença deste patógeno em nossos rebanhos suínos constituindo-se consequentemente em mais um problema de saúde pública.

Abstract in English:

The diarrheic processes caused by pathogenic enterobacteria are of great economicsanitary importance, with limiting effects on cattle raising. In the present study 106 fecal samples from 52 diarrheic and 54 non-diarrheic calves aged 1 to 90 days were analyzed, in arder to determine the occurrence of enterotoxigenic Escherichia coli (ETEC) and Salmonella sp among calves reared in the agreste region of the state of Pernambuco and to determine the behavior of the isolates in response to 16 antimicrobial agents. The strains were cultured in selective media and submitted to biochemical characterization, serologic identification and detection of heat-stable enterotoxin. E. coli was isolated from all 106 calves studied and five of these strains (4,7%) were proclucers of heat-stable enterotoxin. Salmonellasp was isolated from six (5,7%) samples belonging to serovars Dublin, Muenchen and Infantis. The Salmonella strains were highly sensitive to the drngs tested, whereas the E. co/istrains were more resistent to tetracycline (50,9%), sulfonamide (40,6%), streptomycin (35,9%) and ampicillin (22,6%). Particularly outstancling in the general analysis was the multiresistance of E. coli (52,0%), with a predominance of the tetracycline-sulfonamide association. The present results serve as a warning regarding the incliscriminated use of antimicrobial agents. Low percentages of ETEC and Salmonella in calves show the necessity of other diagnostic procedures to clarify the etiology.

• Diarrheia calves Escherichia coli Salmonella sp

Abstract in Portuguese:

Os processos diarréicos acarretados por enterobactérias patogênicas têm sido incriminados como de grande importância econômica-sanitária, desempenhando um papel limitante na criação de bezerros. Com o objetivo de estudar a ocorrência de Escherichia coli enterotoxigênica e Salmonella sp em bezerros no agreste meridional de Pernambuco e o compottamento dos isolados frente aos antimicrobianos, foram analisadas 106 amostras fecais provenientes de 52 bezerros diarréicos e 54 não diarréicos de 1 a 90 dias de idade. O cultivo das amostras foi realizado em meios seletivos com posterior caracterização bioquímica, identificação sorológica e detecção de enterotoxina termoestável. Para verificação do comportamento dos isolados, foram selecionados 16 antimicrobianos. E. coli foi isolada dos 106 bezerros estudados, sendo cinco (4,7%) produtoras de enterotoxina termoestável e, Salmonella isolada de seis (5,7%) amostras pertencentes aos sorovares Dublin, Muenchen e Infantis. As amostras de Salmonella demonstraram uma elevada sensibilidade frente as drogas testadas, enquanto que as de E. coli revelaram maior resistência à tetraciclina (50,9%); sulfonamida (40,6%); estreptomicina (35,9%) e ampicilina (22,6%). No cômputo geral destaca-se a multirresistência de E. coli (52%), predominando a associação tetraciclina-sulfonamida. Face aos resultados auferidos adverte-se quanto ao uso indiscriminado de antimicrobianos. Os baixos percentuais de ETEC e Salinonella salientam a necessidade de outros procedimentos de diagnóstico visando esclarecer a etiologia das diarréias.

• Diarréia bezerros Eschericbia coli Salmonella sp

Abstract in English:

The extent of antigen interference between Yersinia enterocolitica 0:9 and Bmcella spp was evaluated in 245 bovine serum samples divided into groups according to status of immunization against brucellosis, and in 119 swine serum samples. The specimens were submitted to the plate serum agglutination test, tube serum agglutination test and to the Rose Bengal test for Brucella spp, and to tube sérum agglutination for Y. enterocolitica 0:9. The immune response to Y. enterocolitica was demonstrated and considered significant in terms of its possible effects on the interpretation of serological tests for brucellosis. Agglutinating titers were dose or even similar from a quantitative viewpoint. Since in most cases it was not possible to determine precisely the etiologic agent, it is clearly necessary to set up a laboratory method for the diagnosis of brucellosis that will permit the distinction of specific infections.

• Brucellosis Yersiniosis cross-reaction Yersinia enterocolitica 0:9 Brucella spp

Abstract in Portuguese:

O grau de interferência antigênica entre Yersinia enterocolitica 0:9 e Bmcella spp foi analisado em 245 soros de bovinos discriminados quanto ao estado de imunização contra brucelpse, e 119 soros de suínos. Os espécimens foram submetidos às provas de soroaglutinação rápida, soro-aglutinação lenta e antígeno acidificado para Brucella spp e soro-aglutinação lenta para Y. enterocolitica 0:9. A resposta imune a Y. enterocolitica foi evidenciada e considerada significativa em relação aos seus possíveis efeitos na interpretação dos testes sorológicos para brucelose. Os títulos aglutinantes foram aproximados ou mesmo similares do ponto de vista quantitativo. Como não foi possível determinar com precisão, na maioria dos casos, o agente etiológico, é necessário instituir um método laboratorial de diagnóstico de brucelose que permita distinguir as infecções específicas.

• Yersinia enterocolitica Brucella spp reação cruzada bovino suíno